To visualize where cosmids fit into the hierarchy of genetic cloning, review the operational differences below: Vector Type Insert Capacity Origin / Features Primary Application 1 – 10 kb Bacterial plasmid origin Routine cloning, expression Bacteriophage 9 – 23 kb Viral genome fragments Genomic and cDNA screening Cosmid 30 – 45 kb Plasmid + Phage cos sites Genomic library construction BAC (Bacterial Artificial Chromosome) 100 – 300 kb Functional F-plasmid origin Large-scale genome sequencing
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